Prevalence of Malaria and Helicobacter Pylori Coinfection and Associated Sociodemographic Factors among Blood Donors in Douala General Hospital
Watching Djakissam *
Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Southwest Cameroon.
Thelma Epole Agberndifor
Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Southwest Cameroon.
Ndouwe Tissebe Menga Honoré
Department of Microbiology, Haematology, Parasitology and Infectious Diseases, Faculty of Medicine and Biomedical Sciences, University of Garoua, North Cameroon.
Ebude Roda Nzegge
Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Southwest Cameroon.
Zeuko’o Menkem Elisabeth
Department of Biomedical Sciences Faculty of Health Sciences, University of Buea, Southwest Cameroon.
Yimtchi Ngassa Christian Lavoisier
Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Buea, Southwest Cameroon.
Abange William Baiye
Department of Biomedical Sciences Faculty of Health Sciences, University of Buea, Southwest Cameroon and Bacteriology Laboratory, Yaoundé University Teaching Hospital, Yaoundé, Centre Cameroon.
Emmanuel Rode Mengue
Department of Clinical Biology, Douala General Hospital, Littoral, Cameroon and Department of Clinical Biology, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Littoral, Cameroon.
*Author to whom correspondence should be addressed.
Abstract
Background: The malaria parasite Plasmodium is intra-erythrocyte hence can be transmitted by transfusion of any blood component containing infected red blood cells. Helicobacter pylori infection affects more than half of the human population worldwide. Infection remains asymptomatic in most individuals, although some individuals develop acute gastritis, peptic or duodenal ulcers, gastric cancer, and mucosa associated tissue lymphoma. H. pylori infection have been implicated in some haematological manifestations such as anaemia and micronutrient deficiency.
Objective: This research was designed to determine the prevalence of malaria parasitaemia and H. pylori coinfection in association with sociodemographic characteristics among donors in Douala General Hospital.
Methodology: The study was a hospital-based study carried out from January to May 2022. The study population comprised of 106 donors who came to the hospital laboratory for blood donation. A structured questionnaire was used to get demographic and clinical data. Two ml of blood was collected from individuals to prepare a thick and thin blood film for malaria parasite determination and to centrifuge at 3000 rpm for 5 minutes to obtain plasma for H. pylori test strip.
Results and Discussion: The overall prevalence of H. pylori in the study area was 70.8%. The female donors had a prevalence of 72.0%. A greater proportion of blood group O donors had H. pylori than groups A, AB and B but this difference was not significant (P-value = 0.571). The prevalence of malaria parasites infection was 36.8%. Those of blood group A had a higher prevalence rate of 42.3% (11/26). Out of the 106 donors, 23 (21.7%) had malaria and H. pylori coinfection. 68 (64.2%) donors had a mono-infection that is either having malaria mono-infection or H. pylori mono-infection and 15 (14.2%) had no infection. Malaria and H. pylori coinfection was higher in female donors than males (OR = 1.059, 95% Cl 0.359 -3.119, P =0.917), in donors aged below 25 years old (OR = 0.338, 95% Cl 0.075 - 1.535, P =0.160), replacement donors (OR = 1.350, 95% Cl 0.460 – 3.964, P =0.585); blood donors of blood group AB (OR = 0.654, 95% Cl 0.181 – 2.366, P =0.517) and Rhesus negative donors (OR = 1.500, 95% Cl 0.130 – 17.36, P =0.746).
Conclusion: The prevalence of H. pylori infection and Malaria among blood donors in Douala General Hospital were high and coinfection was higher in females than males. Both malaria parasites and H. pylori infections have been implicated in blood parameters such as anaemia and micronutrient deficiency. It is therefore recommended that routine screening for malaria parasites and H. pylori be done in blood banks before transfusion for a high blood quality.
Keywords: Malaria parasites, helicobacter pylori, blood donors, Cameroon
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References
Baye G, Yohannes M. The prevalence of HBV, HCV and malaria parasites among blood donors in Amhala and Tigray regional states. Ethiop. J. Health Dev. 2007;22(1):3-7.
Gelaw B, Mengistu Y. The prevalence of HBV, HCV and malaria parasites among blood donors in Amhara and Tigray regional states. Ethiop J Health Dev. 2008;22(1):1–5.
Diop S, Ndiaye M, Seck M, Chevalier B, Jambou R, Sarr A, et al. [Prevention of transfusion transmitted malaria in endemic area] (in French). Transfus Clin Biol. 2009;16:454–9.
Keshavarz H. Asymptomatic malaria and its challenges in the malaria elimination program in Iran: A systematic review. Journal of Arthropod-Borne Disease. 2012;11(2):172–181.
Kitchen AD, Chiodin PL. Malaria and blood transfusion. Vox Sanguinis. 2006;90 (2):77–84.
Kouitcheu Mabeku LB, Noundjeu Ngamga ML, Leundji H. Potential risk factors and prevalence of helicobacter pylori infection among adult patients with dyspepsia symptoms in Cameroon. BMC Infectious Diseases. 2018;9;18(1):1-1.
Yousryeia AR, Lobna Abdel WA, Rania MMH, Rasha MMA. Helicobacter pylori and its hematological effect. The Egyptian Journal of Internal Medicine. 2019;31:332–342.
Ernst PB, Gold BD. The disease spectrum of Helicobacter pylori: The immunepathogenesis of gastroduodenal ulcer and gastric cancer. Annu Rev Microbiol. 2000;54:615–40.
Owusu-Ofori AK, Betson M, Parry CM, Stothard JR, Bates I. Transfusion-transmitted malaria in Ghana. Clinical Infectious Diseases. 2013 Jun 15;56(12):1735-41.
Ezeonu CM, Adabara NU, Garba SA, Kuta FA, Ewa EE, Oloruntoba PO, Atureta Z. The risk of transfusion transmitted malaria and the need for malaria screening of blood donors in Abuja, Nigeria. African Journal of Clinical and Experimental Microbiology. 2019 May 23;20(3):195-201.
Mogtomo MK, Foko LK, Okoubalimba EA, Enyegue EE, Ngane AR. High risk of transfusion-transmitted malaria (TTM) from student blood donors living in the town of Douala, Cameroon. J Clin Infect Dis Pract. 2016;1(108):2.
Okalla Ebongue C, Ngouadjeu Dongho E, Texier G, Nda Mefo’o JP, Sume Etapelong G, Ayong L, Eboumbou Moukoko CE. Residual risk of transfusion-transmitted malaria infection in a malaria endemic sub-Saharan African setting. Translational Medicine Communications. 2017 Dec; 2: 1-7.
Takem EN, Achidi EA, Ndumbe PM. An update of malaria infection and anaemia in adults in Buea, Cameroon. BMC Res Notes. 2010;3:121.
Noubouossie D, Tagny CT, Same-Ekobo A, Mbanya D. Asymptomatic carriage of malaria parasites in blood donors in Yaoundé. Transf Med. 2012;22:63–7
Erhabor O, Ok O, Awah I, Uko KE and Charles AT. The prevalence of plasmodia parasitaemia among donors in the Niger delta of Nigeria. Trop Doct. 2007;37:32–4.
Okocha EC, Ibeh CC, Ele PU, Ibeh NC. The prevalence of malaria parasitaemia in blood donors in a Nigerian teaching hospital. Journal of Vector Borne Diseases. 2005 Mar 1;42(1):21.
Agboola TF, Ajayi MB, Adeleke MA, Gyang PV. Prevalence of malaria parasite among blood donors in Lagos University Teaching Hospital, Lagos Nigeria. Annals of Biological Research. 2010;1(3):72-5.
Muntaka S, Opoku-Okrah C. The prevalence of malaria parasitaemia and predisposition of ABO blood groups to Plasmodium falciparum malaria among blood donors at a Ghanaian Hospital. AU Journal of Technology. 2013;16(4).
Owusu-Ofori A, Gadzo D, Bates I. Transfusion-transmitted malaria: donor prevalence of parasitaemia and a survey of healthcare worker’s knowledge and practices in a district hospital in Ghana. Malar J. 2016;15(1):234.
Diop S, Ndiaye M, Seck M, Chevalier B, Jambou R, Sarr A, et al. [Prevention of transfusion transmitted malaria in endemic area] (in French). Transfus Clin Biol. 2009;16:454–9.
Oladeinde BH, Omoregie R, Osakue EO, Onaiwu TO. Asymptomatic malaria among blood donors in Benin City Nigeria. Iranian Journal of Parasitology. 2014 Sep;9(3):415.
Uneke CJ, Ogbu O, Nwojiji V. Potential risk of induced malaria by blood transfusion in South-eastern Nigeria. McGill Journal of Medicine: MJM. 2006 Jan;9(1):8.
Mogtomo ML, Fomekong SL, Kuate HF, Ngane AN. Screening of infectious microorganisms in blood banks in Douala (1995-2004). Cahiers d'études et de recherches francophones/Santé. 2009 Jan 1;19(1):3-8.
Khosravi AD, Sirous M, Saki M, Seyed-Mohammadi S, Modares Mousavi SR, Veisi H, Abbasinezhad Poor A. Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran. Journal of International Medical Research. 2021 Dec;49(12):03000605211058870
Sasidharan S, Uyub AM. Prevalence of Helicobacter pylori infection among asymptomatic healthy blood donors in Northern Peninsular Malaysia. Transactions of the Royal Society of Tropical Medicine and Hygiene. 2009 Apr 1;103(4):395-8.
Us D, Hascelik G. Seroprevalence of Helicobacter pylori infection in an asymptomatic Turkish population. J Infect. 1998;37:148-150.
Al-Balushi MS, Al-Busaidi JZ, Al-Daihani MS, Shafeeq MO, Hasson SS. Sero-prevalence of Helicobacter pylori infection among asymptomatic healthy Omani blood donors. Asian Pacific Journal of Tropical Disease. 2013 Apr 1;3(2):146-9.
Khoder G, Muhammad JS, Mahmoud I, Soliman SS, Burucoa C. Prevalence of Helicobacter pylori and its associated factors among healthy asymptomatic residents in the United Arab Emirates. Pathogens. 2019 Apr 1;8(2):44.
Namyalo E, Nyakarahuka L, Afayoa M, Baziira J, Tamale A, Atuhaire GC, Kungu JM. Prevalence of Helicobacter pylori among patients with gastrointestinal tract (GIT) symptoms: A retrospective study at selected Africa air rescue (AAR) clinics in Kampala, Uganda, from 2015 to 2019. Journal of Tropical Medicine. 2021 Nov 8;2021:1-0.
Tadesse E, Daka D, Yemane D, Shimelis T. Seroprevalence of Helicobacter pylori infection and its related risk factors in symptomatic patients in southern Ethiopia. BMC Research Notes. 2014 Dec;7:1-5.
Almarime A, Allafi A, Albshti S, Almrabet W, Alraheem H, Bahroun S, Eshrif A, Hweissa NA, Zaet A, Elazomi A. The prevalence of Helicobacter pylori infections among blood donors in blood bank of zawia teaching hospital and the assessment of some related factors.
Khosravi AD, Sirous M, Saki M, Seyed-Mohammadi S, Modares Mousavi SR, Veisi H, Abbasinezhad Poor A. Associations between seroprevalence of Helicobacter pylori and ABO/rhesus blood group antigens in healthy blood donors in southwest Iran. Journal of International Medical Research. 2021 Dec;49(12):03000605211058870.
Nyanga JLN, Patakinzo CG, Ngum CN, Desdemona NN and Nyanga BY. The influence of Helicobacter pylori infection on malaria parasitaemia among symptomatic patients in Buea, Cameroon. International Journal of Tropical Disease and Health. 2018;31(4):1-7.
Bartonjo G, Oundo J, Ng’ang’a Z. Prevalence and associated risk factors of transfusion transmissible infections among blood donors at Regional Blood Transfusion Center Nakuru and Tenwek Mission Hospital, Kenya. Pan Afr Med J. 2019 Sep 16;34:31.
Malaria factsheet No 94". WHO (March 2014). Achived from the original on 3rd September 2014. Retrieved 28th August 2014.