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Aims: Effects of Vitamin C and Choline chloride supplementation on temperature and some haematological parameters were investigated in Trypanosoma congolense infected rats.
Methodology: A total of twenty five (25) healthy albino rats weighing between 113.5-154.3 g were used for the study. They were randomly divided into groups (A-E) of five rats per group. Group A was uninfected untreated which served as the control, group B was infected untreated. Groups C, D and E were infected treated with Choline chloride, Vitamin C and combination of Choline chloride and Vitamin C respectively.
Results: The mean body temperature was significantly higher (p<0.05) in the infected untreated group on days 21 and 28 post infection, when compared to the infected supplemented treated groups. The mean packed cell volume (PCV) decreased significantly (p <0.05) in the infected untreated group on days 21 and 28 post infection, when compared to the other infected treated groups. The mean haemoglobin concentration (HB) decreased significantly (p <0.05) in the infected untreated group on days 7, 21 and 28 post infection, when compared to the other infected and treated groups. The mean red blood cell counts (RBC) decreased significantly (p <0.05) in the infected untreated group on day 28 post infection compared to the other infected and treated groups. The mean total white blood cell counts (TWBC) decreased significantly (p <0.05) in the infected untreated group on days 14 and 21 post infection compared to the infected treated groups. On day 28 post infection TWBC counts in the group treated with combination of vitamin C and Choline chloride increased (p <0.05) when compared with the other treated groups. There was no significant difference (p>0.05) in all the infected treated groups when compared with the control
Conclusion: It was concluded from the study that combined supplementation of Vitamin C and Choline chloride will be more effective in ameliorating the haematological impacts of trypanosomosis.
Welburn SC, Fevre EM, Coleman PG, Odiit M, Maudlin I. Sleeping sickness: A tale of two diseases. Trends in Parasitology. 2001;17(1):19-24.
Abenga JN, Lawal LA. Implicating role of animal reservoir host in the resurgence of Gambiantrypanosomosis (sleeping sickness). African Journal of Biotechnology. 2005;4:134-137.
Kneeland KM, Skoda SR, Hogsette JA, Li AY, Molina-Ochoa J, Lohmeyer H, Foster JE. A century and a half of research on the stable fly, Stomoxys calcitrans (L.) (Diptera: Muscidae), 1862-2011: An Annotated Bibliography. United States Department of Agriculture, Agricultural Research Service. 2012; ARS-173.
Chretien JP, Smoak BL. African Trypanosomiasis: Changing epidemiology and consequences. Current Infectious Disease Reports. 2005;7:54-60.
Losos GJ, Ikede BO. Review of pathology of diseases in domestic and laboratory animals caused by Trypanosoma congolense, T. vivax, T.brucei, T. rhodesiense and T. gambiense. Journal of Veterinary Pathology. 1972;9:1-17.
Biryomumaisho SE, Katunguka-Rwakishaya E, Rubaire-Akiiki CM. Serum biochemical changes in experimental Trypanosoma congolense and Trypanosoma brucei infection in Small East Africa goats. Veterinarski Arhiv. 2003; 73:167 – 180.
Anosa VO. Haematological and biochemical changes in human and animal trypanosome. Revue d’elevage et de medicine Veterinaire des Pays tropicaux. 1988;41:151–164.
Anosa VO, Isoun TT. Serum proteins, blood and plasma volumes in experimental Trypanosoma vivax infection of sheep and goats. Tropical Animal Health and Production. 1976;8:14-19.
Anene BM, Onah DN. Drug resistance in pathogenic African trypanosomes: What hopes for the future? Veterinary Parasitology. 2001;96:83-100.
Igbokwe IO. Mechanisms of cellular injury in African Trypanosomiasis. Veterinary Bulletin. 1994;64:611-620.
Umar IA, Toh ZA, Igbalajobi FI, Igbokwe IO, Gidado A. The effect of orally administered vitamins c and e on severity of anaemia in Trypanosoma brucei-infected rats. Tropical Veterinarian. 2000; 18:71-77.
Hoet S, Opperdoes B, Brun R, Quetin-Leclercq J. Natural products active against African trypanosomes: A step towards new drugs. Natural Products Reports. 2004;21: 353-364.
Chibale K. Economic drug discovery and rational medicinal chemistry for tropical diseases. Pure and Applied Chemistry. 2005;77:1957-1964.
Umar IA, Toma I, Akombum CA, Nnadi CJ, Mahdi MA, Gidado A, Igbokwe IO, Buratai LB. The role of intraperitoneally administered vitamin C during Trypanosoma congolense infection of rabbits. Global. Journal of Pure and Applied Science. 2007;In press.
Verlhac V, Gabaudan J. The effect of vitamin C on fish health. Saint-Louis Cedex: Centre for research in animal nutrition, Société Chimique Roche, Veterinarian. 2009;6(4):402-408.
Chambial S, Dwivedi S, Shukla KK, John PJ, Sharma P. Vitamin C in disease prevention and cure: An overview. Indian Journal of Clinical Biochemistry. 2013; 28(4):314-328.
Zeisel SH, da Costa KA. Choline: An essential nutrient for public health. Nutrition Reviews. 2009;67(11): 615–23.
Zeisel SH. Choline: Critical role during fetal development and dietary requirements in adults. Annual Revelation Nutrition. 2006; 26:229–250.
Sanders LM, Zeisel SH. Choline: Dietary requirements and role in brain development. Nutrition Today. 2007;42(4): 181–186.
Legros D, Ollivier G, Etchegorry M, Pacquet C, Burri C, Jannin J, Buscher P. Treatment of human African trypanosomiasis-present situation and needs for research and development. The Lancet Infectious Diseases. 2002;2(7):437-440.
Eze JI, Ochike BO. Effect of dietary vitamin E supplementation on Trypanosoma brucei infected mice. Tropical Vetenarian. 2007; 25:18-25.
National Research Council. Guide for the Care and Use of Laboratory Animals. National Academies Press. 2010. Washington DC.
Herbert WJ, Lumsden WHR. Trypanosoma brucei, a rapid “matching” method for estimation of host’s parasitemia. Experimental Parasitology. 1976;40:427-431.
Coles EH. Veterinary clinical pathology (4th Edition). W.B. Sunders Company, Harcourt Brace Jovanovidi, Inc; 1986.
Kachmar JF. Determination of haemoglobin by cyanomethaemoglobin procedure. In: Tietz NW, (Ed) Fundamentals of clinical chemistry. Saunders, Philadelphia. 1970;268–269.
Schalm OW, Jain NC, Caroll EJ. Veterinary haematology, 3rd Edn. Lea and Febiger, Philadelphia. 1975;19–25.
Duncan DB. Multiple range and multiple F test. Biometrics. 1995;11:1-42
Brown C. Trypanosomiasis, African. In: Foreign animal diseases. 7th edition. Boca Raton, F. L: United States Animal Health Association. 2008;405-409.
Zwart D, Brouwer BC, VanderHel W, Van den Akker HN, Versegen MWA. Effect of Trypanosoma vivax infection on body temperature, feed intake and metabolic rate of West African Dwarf goat. Journal of Animal Science. 1991;69:3780-3788.
Stephen LE. Trypanosomiasis- A veterinary perspective. 1st (Eds). pergamon press, Oxford; 1986.
ILRAD. Annual report of the international laboratory for research on animal diseases; 1990. Avilable:http://www.ilri.org/InfoServ/Webpub/Fulldocs/Ilrad90/Trypano.htm#P77_15231 Retrieved on October 17, 2015.
Umar IA, Wuro-Chekke AU, Gidado A, Igbokwe IO. Effects of combined parenteral vitamin C and E administration on the severity of anaemia, hepatic and renal damage in T. brucei infected rabbits. Veterinary Parasitology. 1999;85:43-47.
Igbokwe IO, Umar A, Omage JJ, Ibrahim NDB, Kadima KB, Obagaiye OK, Saror DI, Esievo KAN. Effect of acute Trypanosoma vivax infection on cattle erythrocyte glutathione and susceptibility to in vitro peroxidation. Veterinary Parasitology. 1996;63(3-4):215-224.
Taiwo VO, Olaniyi MO, Ogunsanmi AO. Comparative plasma biochemical changes and susceptibility of erythrocytes to in vitro peroxidation during experimental Trypanosome congolense and T brucei infections in sheep. Israel Journal of Veterinary Medicine. 2003;58:4.
Akanji MA, Adeyemi OS, Oguntoye SO, Sulyman F. Psidium guajava extract reduces trypanosomosis associated lipidperoxidation and raises glutathione concentrations in infected animals. Experimental and Clinical International online Journal. 2009;8:148-154.
Naessens C, Hendy CR, Leaver JD. Effects of nutrition on productivity and health of N’ Dama cows infected with Trypanosoma congolense. Research in Veterinary Science. 2005;52:292-298.
German JB, Traber MG. Nutrients and Oxidation: Actions, Transport, and Metabolism of Dietary Antioxidants. In: Handbook of vitamins. 3rd (Eds). Rucker RB, Suttie JW, McCormick DB, Machlin LJ, Marcel Dekker Inc., New York; 2001.
Zeisel SH. A brief history of choline. Annual Nutrition Metabolism. 2012;61:254-258.
Abubakar A, Iliyasu B, Yusuf AB, Igweh AC, Onyekwelu NA, Shamaki BA, Afolayan DO, Ogbadoyi EO. Antitrypanosomal and haematological effects of selected Nigerian medicinal plants in Wistar rats. Biochemistry. 2005;17:95-99.
Ekanem JT, Yusuf OK. Some biochemical and haematological effects of black seed (Nigella sativa) oil on T. brucei infected rats. African Journal of Biomedical Research. 2008;11:79–85.
Losos GJ. Infectious tropical diseases of domestic animals, Churchill Livingstone Incorporation., New York; 1986.
Murray M, Dexter TM. Anaemia in bovine Africa trypanosomiasis: A review. Actatropica. 1988;45:389-432.